Edited by Donald Ort, University of Illinois at Urbana Champaign, Urbana, IL; received December 10, 2024; accepted January 28, 2025 ...

This paper describes a high resolution cryo-EM structure of an [FeFe] hydrogenase purported to operate via an electron bifurcating mechanism. The study aims to resolve a controversy regarding the site ...

* Data collected from two crystals were used for structure determination.