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C&EN1mon
RNA G-Quadruplex Reprogramming with Guanine-Rich Antisense Oligonucleotides Inhibits Monoamine Oxidase B’s Translation
For transcription, a solution containing 2 μM of the double-stranded DNA, 6.25 mM rNTP, 80 mM HEPES-KOH pH 7.5, 24 mM MgCl 2, 2 mM spermidine, 40 mM DTT, 20 μg/mL pyrophosphatase (Roche) and a ...
Frontiers2y
Decreased RNA polymerase III subunit expression leads to defects in oligodendrocyte development
Introduction: RNA polymerase III (Pol III) is a critical enzymatic complex ... and confirmed that Pol III also plays a critical role in myelinogenesis. Decreasing Pol III subunit expression in ...
Frontiers4y
rDNA Chromatin Activity Status as a Biomarker of Sensitivity to the RNA Polymerase I Transcription Inhibitor CX-5461
Hyperactivation of RNA polymerase I (Pol I) transcription of ribosomal RNA (rRNA) genes (rDNA) is a key determinant of growth and proliferation and a consistent feature of cancer cells. We have ...
eLife5y
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
The Positive Transcription Elongation Factor b (P-TEFb) phosphorylates Ser2 residues of the C-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II and is essential for the ...
eLife9y
Methylation of RNA polymerase II non-consensus Lysine residues marks early transcription in mammalian cells
These messenger RNAs are then used as templates for protein production. In animals, plants and other eukaryotic organisms, an enzyme called RNA polymerase II is responsible for making messenger RNA ...