Fred Hutch and MSK researchers showed that tumors driven by hiccups in RNA processing can't hid from the immune system. In ...

Attribution (BY): Credit must be given to the creator. The study of “resource allocator” bacteriophage T7 RNA polymerase (T7RNAP) has garnered significant interest, particularly for optimizing ...

The cost-effective, easy-to-use T7 expression system has been integral to Escherichia coli protein production since its introduction in 1986. Now, a just-developed enhancement is making it even ...

Protein yield and ease of purification are two important criteria in choosing a system for the expression/ purification of recombinant proteins. The IMPACT (Intein Mediated Purification with an ...

By comparing both strategies, G-ASOs were shown to be more effective and to specifically repress MAOB expression. This research highlights ... and a laboratory-prepared T7 RNA polymerase (2 μL in a ...

RNA-based therapeutics have rapidly emerged over the past decade, offering a new class of medicines that differ significantly from conventional drugs. These therapies can be programmed to target or ...

They were particularly drawn to features of T7 DNA polymerase—derived from the T7 bacteriophage ... scientists mapping the RNome and advance our overallunderstanding of RNA.

DNA Polymerase I, and RNase H. The library fragments were purified on an AMPure XP system (Beckman Coulter, United States) to obtain cDNA fragments with lengths of 250–300 bp. The library was prepared ...

RNA integrity was assessed using the Agilent 2100 Bioanalyzer (#G2943CA, Agilent Technologies), which provides an RNA integrity number (RIN) from microfluidics analysis. The Ambion Whole-Transcript ...

In the drug treated cells, RNA polymerase II was found more frequently ‘stalled’ at positions on the DNA just before a gene starts. This suggests that living cells needs this two-phosphate code system ...