Sera were prepared from the blood samples and stored at −80°C until the assays were performed ... After washing with PBS containing 0.05% Tween 20 (PBS-T) (Nacalai Tesque Inc.), the plates were ...

Briefly, iBMDMs were plated at a density of 1 × 10 5 cells/mL in 96-well plates and exposed to different concentrations ... The equal amounts of proteins were separated by SDS-PAGE gel at 80 V for 0.5 ...

The PathHunter protein complementation assay (DiscoverX) using the PathHunter® HEK 293 CCR7 β-Arrestin cell line was performed according to the manufacturer’s protocol using and read for ...

The slides were denatured on a hot plate (94°C) for 5 min and hybridization was performed ... A stringent wash was performed with 0.5× standard saline citrate at 75°C for 5 min; a PBS/Tween 20 wash ...

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Choose from Red Paper Plate stock illustrations from iStock. Find high-quality royalty-free vector images that you won't find anywhere else. Video Back Videos home Signature collection Essentials ...

Daniella Gray is a Newsweek Family & Parenting Reporter based in London, U.K. Her focus is on family dynamics, childhood development, parenting styles, U.S. education and current trends in family ...

He played in only 22 games with Philadelphia, producing a disappointing -0.3 WAR in 80 plate appearances. As a result, the former All-Star was non-tendered early in the offseason, making him a ...

Last month the city and police began asking residents to make a report to 311 if they knew someone who lived in Quebec with an out-of-province plate. Residents have 90 days to switch after they move.

We found at least two products that market themselves as a so-called 'stealth plates defender' that boasts to illegally make licence numbers undetectable to ANPR cameras. One reflective spray ...