News

C&EN1mon
RNA G-Quadruplex Reprogramming with Guanine-Rich Antisense Oligonucleotides Inhibits Monoamine Oxidase B’s Translation
For transcription, a solution containing 2 μM of the double-stranded DNA, 6.25 mM rNTP, 80 mM HEPES-KOH pH 7.5, 24 mM MgCl 2, 2 mM spermidine, 40 mM DTT, 20 μg/mL pyrophosphatase (Roche) and a ...
Frontiers2y
Decreased RNA polymerase III subunit expression leads to defects in oligodendrocyte development
Introduction: RNA polymerase III (Pol III) is a critical enzymatic complex ... and confirmed that Pol III also plays a critical role in myelinogenesis. Decreasing Pol III subunit expression in ...
Frontiers4y
rDNA Chromatin Activity Status as a Biomarker of Sensitivity to the RNA Polymerase I Transcription Inhibitor CX-5461
Hyperactivation of RNA polymerase I (Pol I) transcription of ribosomal RNA (rRNA) genes (rDNA) is a key determinant of growth and proliferation and a consistent feature of cancer cells. We have ...
eLife5y
Tyr1 phosphorylation promotes phosphorylation of Ser2 on the C-terminal domain of eukaryotic RNA polymerase II by P-TEFb
The Positive Transcription Elongation Factor b (P-TEFb) phosphorylates Ser2 residues of the C-terminal domain (CTD) of the largest subunit (RPB1) of RNA polymerase II and is essential for the ...