Henry Erlich, Children’s Hospital Oakland Research Institute Still dedicated to making the technique work, Cetus Corporation scientists David Gelfand and Susanne Stoffel purified a thermostable DNA ...

DNA analysis is crucial for identifying individuals and their relatives, using technologies like PCR, electrophoresis, and ...

The technique, now a staple of every molecular biology lab, uses the thermostable Taq DNA polymerase to extend short single-stranded synthetic primers using the target DNA or cDNA as a template ...

The team focused on T7 DNA polymerase’s thioredoxin binding domain (TBD), which has a structure similar to that of most thermostable polymerases, including Taq. Thioredoxin, which is ubiquitous in ...

3| Add 5.5 μl of genomic DNA at 10 ng/μl to the master mix (Step 1) and mix thoroughly. 4| Distribute 48 μl to each of ten reaction tubes. 5| To each of the ten reaction tubes, add 1 μl of ...