The standard eukaryotic cell cycle consists of four phases: G1, S, G2, and M phases. There are three phase transitions in a cell cycle: G1/S to initiate DNA synthesis, G2/M to enter mitosis ...

The stain can be applied to live, detergent-permeabilized or fixed cells for quantification of their distribution among the three main phases of the cell cycle (G 0 /G 1, S and G 2 /M).

When looking at cells with a microscope, the length of different stages of the cell cycle can be estimated using the formula: \(\text{Length of time in phase}=\) \(\frac{\text{observed number of ...